3 | Development of perivascular macrophages in the endothelial niche

The roles of perivascular macrophages and meningeal macrophages in the CNS are still poorly understood. Thus, we will study the dynamics of brain macrophage development and their interactions with endothelial cells by investigating their temporal expression profiles with single-cell RNA-seq. RNA profiles of brain pvMΦ and mnMΦ will be compared to myeloid progenitors from the yolk sac, microglia and brain endothelial cells.

Publications

• Jordão MJC, Sankowski R, Brendecke SM, Sagar, Locatelli G, Tai Y-H, Tay TL, Schramm E, Armbruster S, Hagemeyer N, Groß O, Mai D, Çiçek Ö, Falk T, Kerschensteiner M, Grün D and Prinz M. Single-cell profiling identifies myeloid cell subsets with distinct fates during neuroinflammation. Science. 2019;363:eaat7554.
• Masuda T, Sankowski R, Staszewski O, Bottcher C, Amann L, Scheiwe C, Nessler S, Kunz P, van Loo G, Coenen VA, Reinacher PC, Michel A, Sure U, Gold R, Priller J, Stadelmann C and Prinz M. Spatial and temporal heterogeneity of mouse and human microglia at single-cell resolution. Nature. 2019;566:388-392.
• Tay TL, Mai D, Dautzenberg J, Fernandez-Klett F, Lin G, Sagar, Datta M, Stempfl T, Ardura-Fabergat A, Staszewski O, Margineau A, Sporbert A, Steinmetz LM, Pospisilik JA, Jung S, Priller J, Grün D, Ronneberger O, Prinz M: A new fate mapping system reveals context-dependent random or clonal expansion of microglia. Nat Neurosci, 2017;20:793-803.
• Goldmann T, Wieghofer P, Jordao MJ, Prutek F, Hagemeyer N, Frenzel K, Amann L, Staszewski O, Kierdorf K, Krüger M, Locatelli G, Hochgerner H, Zeiser R, Epelman S, Geismann F, Priller J, Rossi FM, Bechmann I, Kerschensteiner M, Linnarsson S, Jung S, Prinz M. Origin, fate and dynamics of macrophages at central nervous system interfaces. Nat Immunol 2016;17:797-805.
• Blank T, Detje C, Spieß A, Hagemeyer N, Brendecke S, Wolfart J, Staszewski O, Zöller T, Papageorgiou I, Schneider J, Paricio-Montesinos R, Eisel ULM, Manahan-Vaughan D, Jansen S, Lienenklaus S, Lu B, Imai Y, Müller M, Goelz SE, Baker DP, Schwaninger M, Kann O, Heikenwalder M, Kalinke U, Prinz M. Brain endothelial- and epithelial-specific interferon receptor chain 1 drives virus-induced sickness behaviour and cognitive impairment. Immunity 2016;44:901-912.

Potential secondments

BOEHRINGER (Sebastian Kreuz), month 19-21; UNIBERN (Britta Engelhardt), month 27-28; WWU (Lydia Sorokin), month 32-33