4 | Chemoproteomic technologies for the investigation of endothelial-macrophage interactions

To characterise the endothelial niche, in which macrophages develop, we will employ chemoproteomic technologies. The Cell Surface Capture technology permits quantitative analysis of proteins on the cell surface, the so-called surfaceome. We will apply this approach to compare the basolateral and apical surfaceome of brain endothelial cells in vitro. Building on these results, we will investigate how myeloid cells perturb the polarity of the surfaceome when added to the in vitro BBB models.


  • Glasgow HL, Whitney MA, Gross LA, Friedman B, Adams SR, Crisp JL, Hussain T, Frei AP, Novy K, Wollscheid B, Nguyen QT, Tsien RY. Laminin targeting of a peripheral nerve-highlighting peptide enables degenerated nerve visualization. Proc Natl Acad Sci U S A 2016; 113:12774-12779.
  • Bausch-Fluck D, Hofmann A, Bock T, Frei AP, Cerciello F, Jacobs A, Moest H, Omasits U, Gundry RL, Yoon C, Schiess R, Schmidt A, Mirkowska P, Härtlová A, Van Eyk JE, Bourquin JP, Aebersold R, Boheler KR, Zandstra P, Wollscheid B. A mass spectrometric-derived cell surface protein atlas. PLoS One 2015;10:e0121314.
  • DeVeale B, Bausch-Fluck D, Seaberg R, Runciman S, Akbarian V, Karpowicz P, Yoon C, Song H, Leeder R, Zandstra PW, Wollscheid B, van der Kooy D. Surfaceome profiling reveals regulators of neural stem cell function. Stem Cells 2014;32:258-268.
  • Frei AP, Jeon OY, Kilcher S, Moest H, Henning LM, Jost C, Plückthun A, Mercer J, Aebersold R, Carreira EM, Wollscheid B. Direct identification of ligand-receptor interactions on living cells and tissues. Nat Biotechnol 2012;30:997-1001.
  • Wollscheid B, Bausch-Fluck D, Henderson C, O'Brien R, Bibel M, Schiess R, Aebersold R, Watts JD. Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins. Nat Biotechnol 2009;27:378-386.

Potential secondments

DUALSYSTEMS (Paul Helbling), month 8-10; MIM (Paul Vulto), month 16-17; UNIBERN (Britta Engelhardt), month 20; UKLFR (Marco Prinz), month 28

Host Organisation:
Institute of Molecular Systems Biology, Eidgenössische Technische Hochschule Zürich, Switzerland