13 | Brain endothelial signaling and its regulation by myeloid cells

The project will focus on the dynamics of brain endothelial calcium signaling and its regulation under ischemic conditions with or without microglia. The candidate will use in vitro and in vivo methods to investigate intracellular signaling cascades in brain endothelial cells, including sophisticated methods like 2-photon microscopy and super-resolution imaging in mouse models of stroke and impaired cerebrovascular reactivity.

Publications

• Müller-Fielitz H, Stahr M, Bernau M, Richter M, Krajka V, Benzin A, Wenzel J, Kalies K, Heuer H, Mittag J, Offermanns S, Schwaninger M (2017) Tanycytic feedback mechanism in the hypothalamic pituitary thyroid axis. Nat Comm Sep 07; 8:484
• Assmann JC, Müller K, Wenzel J, Walther T, Brands J, Thornton P, Allan SM, Schwaninger M (2017) Isolation and Cultivation of Primary Brain Endothelial Cells from Adult Mice. BioProtocols 20:7(10)
• Dogbevia G, Töllner K, Körbelin J, Bröer S, Ridder DA, Grasshoff H, Brandt C, Wenzel J, Straub B, Trepel M, Löscher W, Schwaninger M (2017) Gene Therapy Decreases Seizures in a Model of Incontinentia Pigmenti. Ann Neurol 82(1):93-104
• Körbelin J, Dogbevia D, Michelfelder S, Ridder DA, Hunger A, Wenzel J, Seismann H, Lampe M, Pasparakis M, Kleinschmidt JA, Schwaninger M, Trepel M (2016) A new, target-specific adeno-associated viral gene therapy vector for neurovascular diseases. Embo Mol Med 8(6):609-25
• Ridder DA,* Wenzel J*, Müller K, Töllner K, Tong X-K, Assmann JC, Stroobants S, Weber T, Niturad C, Fischer L, Lembrich B, Wolburg H, Grand’Maison M, Papadopoulos P, Korpos E, Truchetet F, Rades D, Sorokin LM, Schmidt-Supprian M, Bedell BJ, Pasparakis M, Balschun D, D’Hooge R, Löscher W, Hamel E, Schwaninger M (2015) Brain endothelial TAK1 and NEMO safeguard the neurovascular unit.  J Exp Med 212 (10): 1529 (*shared First-Authorship)

Potential secondments

IRFMN (Maria-Grazia De Simoni), months 12-14; IEMHAS (Àdàm Dénes), months 19-21.